Gene description for CD63
Gene name CD63 molecule
Gene symbol CD63
Other names/aliases AD1
HOP-26
ME491
MLA1
OMA81H
Pltgp40
TSPAN30
Species Homo sapiens
 Experiment description of studies that identified CD63 in Cervical cancer cells

1
Experiment ID1289
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 19912576   
OrganismHomo sapiens
Experiment descriptionHIV Nef is secreted in exosomes and triggers apoptosis in bystander CD4+ T cells.
AuthorsLenassi M, Cagney G, Liao M, Vaupotic T, Bartholomeeusen K, Cheng Y, Krogan NJ, Plemenitas A, Peterlin BM.
Journal name Traffic.
Publication year2010
SampleCervical cancer cells
Sample nameHeLa.CIITA cells with Nef-GFP
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK EV100021: EV-METRIC:33%, 25%

2
Experiment ID1592
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
immuno-EM
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - 200K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Immuno-EM
EV-TRACK -

3
Experiment ID1542
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 33767144   
OrganismHomo sapiens
Experiment descriptionQuantitative characterization of extracellular vesicle uptake and content delivery within mammalian cells
AuthorsBonsergent E, Grisard E, Buchrieser J, Schwartz O, Théry C, Lavieu G.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID1593
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - CD63 pull-down fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID1594
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - CD63 flow-through fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID1595
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - CD9 pull-down fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID1596
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - CD9 flow-through fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

8
Experiment ID1597
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - IgG control flow-through fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

9
Experiment ID1598
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - mixed conditioned media - IgG control flow-through fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

10
Experiment ID1599
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - mixed conditioned media - CD63 pull-down fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

11
Experiment ID1601
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - mixed conditioned media - CD9 pull-down fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

12
Experiment ID1602
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - mixed conditioned media - CD9 flow-through fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

13
Experiment ID1604
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - 200K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

14
Experiment ID1606
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - 200K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

15
Experiment ID1619
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - 200K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

16
Experiment ID1620
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - 200K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

17
Experiment ID1621
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - 200K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

18
Experiment ID2638
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35649370   
OrganismHomo sapiens
Experiment descriptionRab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells
AuthorsMatsui T, Sakamaki Y, Nakashima S, Fukuda M.
Journal name Cell Rep
Publication year2022
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsDifferential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

19
Experiment ID2639
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35649370   
OrganismHomo sapiens
Experiment descriptionRab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells
AuthorsMatsui T, Sakamaki Y, Nakashima S, Fukuda M.
Journal name Cell Rep
Publication year2022
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsDifferential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

20
Experiment ID2640
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35649370   
OrganismHomo sapiens
Experiment descriptionRab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells
AuthorsMatsui T, Sakamaki Y, Nakashima S, Fukuda M.
Journal name Cell Rep
Publication year2022
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsDifferential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

21
Experiment ID2641
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35649370   
OrganismHomo sapiens
Experiment descriptionRab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells
AuthorsMatsui T, Sakamaki Y, Nakashima S, Fukuda M.
Journal name Cell Rep
Publication year2022
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsDifferential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

22
Experiment ID2840
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35058437   
OrganismHomo sapiens
Experiment descriptionCirculating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2
AuthorsEl-Shennawy L, Hoffmann AD, Dashzeveg NK, McAndrews KM, Mehl PJ, Cornish D, Yu Z, Tokars VL, Nicolaescu V, Tomatsidou A, Mao C, Felicelli CJ, Tsai CF, Ostiguin C, Jia Y, Li L, Furlong K, Wysocki J, Luo X, Ruivo CF, Batlle D, Hope TJ, Shen Y, Chae YK, Zhang H, LeBleu VS, Shi T, Swaminathan S, Luo Y, Missiakas D, Randall GC, Demonbreun AR, Ison MG, Kalluri R, Fang D, Liu H.
Journal name Nat Commun
Publication year2022
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

23
Experiment ID2841
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35058437   
OrganismHomo sapiens
Experiment descriptionCirculating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2
AuthorsEl-Shennawy L, Hoffmann AD, Dashzeveg NK, McAndrews KM, Mehl PJ, Cornish D, Yu Z, Tokars VL, Nicolaescu V, Tomatsidou A, Mao C, Felicelli CJ, Tsai CF, Ostiguin C, Jia Y, Li L, Furlong K, Wysocki J, Luo X, Ruivo CF, Batlle D, Hope TJ, Shen Y, Chae YK, Zhang H, LeBleu VS, Shi T, Swaminathan S, Luo Y, Missiakas D, Randall GC, Demonbreun AR, Ison MG, Kalluri R, Fang D, Liu H.
Journal name Nat Commun
Publication year2022
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
ELISA
EV-TRACK -

24
Experiment ID1607
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa - CD63 pull-down fraction
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

25
Experiment ID1614
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34282141   
OrganismHomo sapiens
Experiment descriptionSpecificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9
AuthorsMathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C.
Journal name Nat Commun
Publication year2021
SampleCervical cancer cells
Sample nameHeLa
Isolation/purification methodsCentrifugal ultrafiltration
Immunoaffinity capture
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

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